Coding

Part:BBa_K3712020:Design

Designed by: Yifan Li   Group: iGEM21_HUST2-China   (2021-10-20)


Wild-type TLR2 antagonist


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 178


Design Notes

The first is to shorten the beta loop to prevent triacylated lipoproteins from missing the target. Secondly, the hydrophobicity of the hydrophobic pocket was enhanced to improve the binding ability of lipoprotein. In this way, we expect to obtain an effective TLR2 antagonist with significant anti-inflammatory effects.


Source

This gene fragment was synthesized in GenScript.

References